PXD000005-4
PXD000005 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic screen reveals Fbw7 as a modulator of the NF-kB pathway |
| Description | Proteomics on HCT116 cells. 3 samples wild-type, 3 samples Fbw7 KO. Subcellular fractionation to cytoplasmic and nuclear fractions. Proteins were reduced with DTT, alkylated with iodoacetamide, digested with trypsin, and labelled with TMT6-plex: 126,127,128 - wildtype; 129,130,131 - Fbw7 KO. Sample separation by high resolution isoelectric focusing at peptide level, whereafter 72 fractions were loaded onto reversed phase LC-MSMS |
| HostingRepository | PRIDE |
| AnnounceDate | 2012-10-03+01:00 |
| AnnouncementXML | Submission_2012-10-26_13:01:14.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rui Branca |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | phosphorylated residue; iodoacetamide derivatized residue; monohydroxylated residue; TMT6plex-126 reporter+balance reagent acylated residue |
| Instrument | LTQ Orbitrap Velos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2012-06-29 03:03:15 | ID requested | |
| 1 | 2012-06-29 03:50:02 | announced | |
| 1 | 2014-07-24 08:12:15 | announced | |
| 2 | 2012-06-29 04:33:50 | announced | missing |
| 3 | 2012-06-29 04:36:58 | announced | missing |
| ⏵ 4 | 2012-08-13 01:54:50 | announced | missing |
| 5 | 2012-10-03 02:27:01 | announced | missing |
| 6 | 2012-10-26 13:01:14 | announced | missing |
| 7 | 2023-11-01 09:05:00 | announced | 2023-11-01: Updated project metadata. |
| 8 | 2023-11-01 09:16:00 | announced | 2023-11-01: Updated project metadata. |
Publication List
| Arabi A, Ullah K, Branca RM, Johansson J, Bandarra D, Haneklaus M, Fu J, Ari, ë, s I, Nilsson P, Den Boer ML, Pokrovskaja K, Grand, é, r D, Xiao G, Rocha S, Lehti, ö J, Sangfelt O, B pathway. Nat Commun, 3():976(2012) [pubmed] |
Keyword List
| submitter keyword: LC-MSMS Orbitrap human colon epithelium Fbw7 HiRIEF |
Contact List
| Rui Branca | |
|---|---|
| contact affiliation | Clinical Proteomics Unit, Dep. of Oncology-Pathology |
| contact email | rui.mamede-branca@ki.se |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2012/06/PXD000005 |
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