<<< Full experiment listing

RPXD034763-1

RPXD034763 is container for one or more analyses. The general container metadata is provided below and the table under Dataset History provides links to the various reanalyses have been provided (if any) in this container.

Reanalysis Container Summary
TitleJPST000090-1 Extended Coverage of Singly and Multiply Phosphorylated Peptides from a Single Titanium Dioxide Microcolumn [Reanalysis: JPST000090]
DescriptionWe developed a novel approach to enlarge phosphoproteome coverage by selective elution depending on the number of phosphoryl group of peptides from a single titanium dioxide (TiO2) microcolumn using hydrophilic interaction chromatography (HILIC). In this approach, acidic methylphosphonate buffer including organic solvent is used for selective elution of singly phosphorylated peptides from an aliphatic hydroxy acid-modified metal oxide chromatography (HAMMOC) microcolumn and basic elution conditions with phosphate, ammonium hydroxide, and pyrrolidine are then employed for eluting multiply phosphorylated peptides retained by the HAMMOC microcolumn. Finally, we successfully identified 11 300 nonredundant phosphopeptides from triplicate analyses of 100 μg of HeLa cell lysates using this approach. This simple strategy made it possible to accomplish comprehensive and efficient phosphoproteome analysis from limited sample amounts loaded onto a single HAMMOC microcolumn without additional fractionation or enrichment approaches. [Original project description]
HostingRepositoryjPOST
AnnounceDate2023-04-01
AnnouncementXMLSubmission_2023-04-01_00:40:43.244.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/RPXD034763
ReviewLevelNon peer-reviewed dataset
DatasetOriginReanalysis of JPST000090
RepositorySupportSupported dataset by repository
PrimarySubmitterjPOST reanalysis
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
InstrumentTripleTOF 5600
Dataset History
ReanalysisRevisionDatetimeStatusTitle / ChangeLog Entry
02022-06-20 17:58:56ID requested
012023-04-01 00:40:43announcedJPST000090-1 Extended Coverage of Singly and Multiply Phosphorylated Peptides from a Single Titanium Dioxide Microcolumn [Reanalysis: JPST000090]
Publication List
no publication
Keyword List
submitter keyword: reanalysis, phosphoproteomics, HAMMOC, TiO2, HILIC
Contact List
Yasushi Ishihama
lab head
jPOST reanalysis
contact affiliationjPOST
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001764/