RPXD034682 is container for
one or more analyses. The general container metadata is provided below and the table under Dataset History provides links to the various reanalyses have been provided (if any) in this container.
Reanalysis Container Summary
Title | JPST000234-1 Identifications of protein kinase A substrates with quantitative phosphoproteomics and primary-sequence based scoring.(H-89) [Reanalysis: JPST000234] |
Description | Protein kinase A (PKA) is a serine/threonine kinase who plays essential roles in the complex signalling pathways. To better understand the functionalities, it is necessary to elucidate direct interplays between PKA and their substrates in living system. In order to screen them in a high-throughput manner, we firstly quantified the change of phosphoproteome in the cells of which PKA activity was perturbed by drag stimulations. LC-MS/MS analysis identified 2,755 and 3,191 phosphopeptides from either experiments with activator or inhibitor of PKA. To exclude indirect targets of PKA, we built the simple model to characterize the kinase sequential specificity to its substrate recognitions based on the known kinase-substrate relationships. Finally, by applying the metric to the sites of which phosphorylation were changed due to the drug stimulations, we identified 29 reliable candidates of PKA targeting residues in living cells including 8 known substrates. Moreover, 18 sites out of them were confirmed to be site-specifically phosphorylated in vitro. Altogether this study proposed confident list of PKA substrate and expanded the knowledge of PKA signalling network. [Original project description] |
HostingRepository | jPOST |
AnnounceDate | 2023-03-25 |
AnnouncementXML | Submission_2023-03-25_06:26:06.089.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/RPXD034682 |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Reanalysis of JPST000234 |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | jPOST reanalysis |
SpeciesList | scientific name: HeLa cell; NCBI TaxID: CLO:0003684; |
ModificationList | TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal; S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine |
Instrument | Q Exactive |
Dataset History
Reanalysis | Revision | Datetime | Status | Title / ChangeLog Entry |
| 0 | 2022-06-19 17:28:55 | ID requested | |
0 | 1 | 2023-03-25 06:26:06 | announced | JPST000234-1 Identifications of protein kinase A substrates with quantitative phosphoproteomics and primary-sequence based scoring.(H-89) [Reanalysis: JPST000234] |
Publication List
Keyword List
submitter keyword: reanalysis, H-89, HeLa, TMT, PKA |
Contact List
Yasushi Ishihama |
lab head | |
jPOST reanalysis |
contact affiliation | jPOST |
dataset submitter | |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001715/ |