RPXD034322 is container for
one or more analyses. The general container metadata is provided below and the table under Dataset History provides links to the various reanalyses have been provided (if any) in this container.
Reanalysis Container Summary
Title | JPST000693 An extended PAXT connection is required for turnover of nuclear polyadenylated RNA in human cells [Reanalysis: JPST000693] |
Description | Recruitment of the human ribonucleolytic RNA exosome to nuclear polyadenylated (pA+) RNA is facilitated by the Poly(A) Tail eXosome Targeting (PAXT) connection. Besides its core dimer, formed by the exosome co-factor MTR4 and the ZFC3H1 protein, the PAXT connection remains poorly defined. By characterizing nuclear pA+-RNA bound proteomes as well as MTR4-ZFC3H1 containing complexes in conditions favoring PAXT assembly, we here uncover
three additional proteins required for PAXT function: ZC3H3, RBM26/RBM27 and the known PAXT-associated protein, PABPN1. The zinc-finger protein ZC3H3 interacts directly with MTR4-ZFC3H1 and loss of either identified PAXT
component results in the accumulation of PAXT substrates. Collectively, our results establish new factors involved in the turnover of nuclear pA+ RNA and suggest that these are limiting for PAXT activity.
[Original project description] |
HostingRepository | jPOST |
AnnounceDate | 2023-03-25 |
AnnouncementXML | Submission_2023-03-25_06:40:58.538.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/RPXD034322 |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Reanalysis of JPST000693 |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | jPOST reanalysis |
SpeciesList | scientific name: HEK293; NCBI TaxID: CLO:0001230; |
ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; unknown modification |
Instrument | Q Exactive |
Dataset History
Reanalysis | Revision | Datetime | Status | Title / ChangeLog Entry |
| 0 | 2022-06-05 06:19:46 | ID requested | |
0 | 1 | 2023-03-25 06:40:58 | announced | JPST000693 An extended PAXT connection is required for turnover of nuclear polyadenylated RNA in human cells [Reanalysis: JPST000693] |
Publication List
Keyword List
submitter keyword: reanalysis, nuclear RNA exosome, RNA degradation, the PAXT connection, ZC3H3, RBM26, RBM27, PABPN1 |
Contact List
Yasushi Ishihama |
lab head | |
jPOST reanalysis |
contact affiliation | jPOST |
dataset submitter | |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001652/ |