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RPXD034322

RPXD034322 is container for one or more analyses. The general container metadata is provided below and the table under Dataset History provides links to the various reanalyses have been provided (if any) in this container.

Reanalysis Container Summary

Title	JPST000693 An extended PAXT connection is required for turnover of nuclear polyadenylated RNA in human cells [Reanalysis: JPST000693]
Description	Recruitment of the human ribonucleolytic RNA exosome to nuclear polyadenylated (pA+) RNA is facilitated by the Poly(A) Tail eXosome Targeting (PAXT) connection. Besides its core dimer, formed by the exosome co-factor MTR4 and the ZFC3H1 protein, the PAXT connection remains poorly defined. By characterizing nuclear pA+-RNA bound proteomes as well as MTR4-ZFC3H1 containing complexes in conditions favoring PAXT assembly, we here uncover three additional proteins required for PAXT function: ZC3H3, RBM26/RBM27 and the known PAXT-associated protein, PABPN1. The zinc-finger protein ZC3H3 interacts directly with MTR4-ZFC3H1 and loss of either identified PAXT component results in the accumulation of PAXT substrates. Collectively, our results establish new factors involved in the turnover of nuclear pA+ RNA and suggest that these are limiting for PAXT activity. [Original project description]
HostingRepository	jPOST
AnnounceDate	2023-03-25
AnnouncementXML	Submission_2023-03-25_06:40:58.538.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/RPXD034322
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Reanalysis of JPST000693
RepositorySupport	Supported dataset by repository
PrimarySubmitter	jPOST reanalysis
SpeciesList	scientific name: HEK293; NCBI TaxID: CLO:0001230;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; unknown modification
Instrument	Q Exactive

Dataset History

Reanalysis	Revision	Datetime	Status	Title / ChangeLog Entry
	0	2022-06-05 06:19:46	ID requested
0	1	2023-03-25 06:40:58	announced	JPST000693 An extended PAXT connection is required for turnover of nuclear polyadenylated RNA in human cells [Reanalysis: JPST000693]

Publication List

no publication

no publication

Keyword List

submitter keyword: reanalysis, nuclear RNA exosome, RNA degradation, the PAXT connection, ZC3H3, RBM26, RBM27, PABPN1

submitter keyword: reanalysis, nuclear RNA exosome, RNA degradation, the PAXT connection, ZC3H3, RBM26, RBM27, PABPN1

Contact List

Yasushi Ishihama
lab head
jPOST reanalysis
contact affiliation	jPOST
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001652/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001652/