PXD051359

PXD051359 is an original dataset announced via ProteomeXchange.

Title	Targeting high circDNA2v levels in colorectal cancer induces cellular senescence and elicits an anti-tumor secretome
Description	The efficacy of immunotherapy against colorectal cancer (CRC) is impaired by insufficient immune cell recruitment into the tumor microenvironment. Our study shows that targeting circDNA2v, a circular RNA commonly overexpressed in CRC, can be exploited to elicit cytotoxic T-cell recruitment. CircDNA2v functions through binding to IGF2BP3, preventing its ubiquitination, and prolonging IGF2BP3 half-life, which in turn sustains the mRNA level of the protooncogene c-Myc. Targeting circDNA2v by gene silencing downregulates c-Myc to concordantly induce tumor cell senescence and the release of proinflammatory mediators. Production of CXCL10 and IL-9 by CRC cells is elicited through JAK-STAT1 signaling, in turn promoting the chemotactic and cytolytic activities of CD8+ T cells. Clinical evidence associates increased circDNA2v expression in CRC tissues with reduction in CD8+ T-cell infiltration and worse outcomes. The regulatory relationship between circDNA2v, cellular senescence and tumor infiltrating lymphocytes thus provides a rationale approach for improving immunotherapy in CRC.
HostingRepository	iProX
AnnounceDate	2024-04-11
AnnouncementXML	Submission_2024-04-11_01:42:27.351.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuang Wu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-11 01:41:58	ID requested
⏵ 1	2024-04-11 01:42:27	announced

Dataset with its publication pending

submitter keyword: circular RNA, circDNA2v, IGF2BP3, c-Myc, senescence, CXCL10, IL-9, CD8+T cells

Hao Gu
contact affiliation	Anhui Medical University
contact email	guhao@ahmu.edu.cn
lab head
Shuang Wu
contact affiliation	Anhui Medical University
contact email	wushuang1994326@126.com
dataset submitter

iProX dataset URI