PXD051139-1
PXD051139 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | A “One-step” Strategy for the Global Characterization of Core-Fucosylated Glycoproteome |
Description | Core fucosylation, a special type of N-linked glycosylation, is important in tumor proliferation, invasion, metastatic potential, and therapy resistance. However, the core-fucosylated glycoproteome has not been extensively profiled due to the low abundance and poor ionization efficiency of glycosylated peptides. Here, a “one-step” strategy has been described for protein core-fucosylation characterization in biological samples. Core-fucosylated peptides can be selectively labeled with a glycosyl-ated probe, which is linked with a temperature-sensitive poly(N-isopropylacrylamide) polymer (PNIPAM), by mutant endo-glycosidase (EndoF3-D165A). The labeled probe can be further removed by wild-type endoglycosidase (EndoF3) in a trace-less manner for mass spectrometry (MS) analysis. The feasibility and effectiveness of the “one-step” strategy are evaluated in the bovine serum albumin (BSA) spiked with standard core-fucosylated peptides, H1299, and Jurkat cell lines. The “one-step” strategy is then employed to characterize core-fucosylated sites in human lung adenocarcinoma, resulting in the identifica-tion of 2494 core-fucosylated sites distributed on 1176 glycoproteins. Further data analysis reveals that 196 core-fucosylated sites are significantly upregulated in tumors, which may serve as potential drug development targets or diagnos-tic biomarkers. Together, this “one-step” strategy has great potential for use in global and in-depth analysis of core-fucosylated glycoproteome to promote its mechanism research. |
HostingRepository | iProX |
AnnounceDate | 2024-04-02 |
AnnouncementXML | Submission_2024-04-01_19:29:17.983.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yuqiu Wang |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | fucosylated |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-04-01 19:28:55 | ID requested | |
⏵ 1 | 2024-04-01 19:29:18 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Core Fucosylation, One-step |
Contact List
Hu Zhou | |
---|---|
contact affiliation | Shanghai Institute of Materia Medica |
contact email | zhouhu@simm.ac.cn |
lab head | |
Yuqiu Wang | |
contact affiliation | Shanghai Institute of Materia Medica |
contact email | 995752924@qq.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |