PXD051139

PXD051139 is an original dataset announced via ProteomeXchange.

Title	A “One-step” Strategy for the Global Characterization of Core-Fucosylated Glycoproteome
Description	Core fucosylation, a special type of N-linked glycosylation, is important in tumor proliferation, invasion, metastatic potential, and therapy resistance. However, the core-fucosylated glycoproteome has not been extensively profiled due to the low abundance and poor ionization efficiency of glycosylated peptides. Here, a “one-step” strategy has been described for protein core-fucosylation characterization in biological samples. Core-fucosylated peptides can be selectively labeled with a glycosyl-ated probe, which is linked with a temperature-sensitive poly(N-isopropylacrylamide) polymer (PNIPAM), by mutant endo-glycosidase (EndoF3-D165A). The labeled probe can be further removed by wild-type endoglycosidase (EndoF3) in a trace-less manner for mass spectrometry (MS) analysis. The feasibility and effectiveness of the “one-step” strategy are evaluated in the bovine serum albumin (BSA) spiked with standard core-fucosylated peptides, H1299, and Jurkat cell lines. The “one-step” strategy is then employed to characterize core-fucosylated sites in human lung adenocarcinoma, resulting in the identifica-tion of 2494 core-fucosylated sites distributed on 1176 glycoproteins. Further data analysis reveals that 196 core-fucosylated sites are significantly upregulated in tumors, which may serve as potential drug development targets or diagnos-tic biomarkers. Together, this “one-step” strategy has great potential for use in global and in-depth analysis of core-fucosylated glycoproteome to promote its mechanism research.
HostingRepository	iProX
AnnounceDate	2024-04-02
AnnouncementXML	Submission_2024-04-01_19:29:17.983.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuqiu Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	fucosylated
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-01 19:28:55	ID requested
⏵ 1	2024-04-01 19:29:18	announced

Dataset with its publication pending

submitter keyword: Core Fucosylation, One-step

Hu Zhou
contact affiliation	Shanghai Institute of Materia Medica
contact email	zhouhu@simm.ac.cn
lab head
Yuqiu Wang
contact affiliation	Shanghai Institute of Materia Medica
contact email	995752924@qq.com
dataset submitter

iProX dataset URI