PXD050426-1

PXD050426 is an original dataset announced via ProteomeXchange.

Title	Multiplexed Proteome Profiling of Targeted Protein Degradation With Data-Independent Acquisition-Based Mass Spectrometry
Description	Targeted protein degradation (TPD) has become a powerful and promising tool that can effective trigger the degradation of specific disease-related proteins utilizing the endogenous pathways of the organism. However, the global proteomic profiling of TPD with multiple dimensions remains lacking since traditional methods of protein quantification are low-throughput and time-consuming. Mass spectrometry (MS)-based quantitative proteomics have been used in TPD research. The quantification with isobaric mass tagging has the problems such as preference for high-abundance species, high cost and ratio compression effects. Here, we employed data-independent acquisition (DIA) MS with multiplexed proteome profiling of TPD, termed DIA-MPT, to implement the label-free proteomic quantitation with satisfactory reproducibility and high accuracy. We demonstrated the power of DIA-MPT for comprehensive profiling of proteolysis targeting chimera (PROTAC) degraders of bromodomain and extra-terminal (BET) and signal transducer and activator of transcription 3 (STAT3) with pharmacodynamic response and drug sensitivity analysis. Consequently, DIA-MPT has potential as a new platform for high-throughput, flexible, and comprehensive multidimensional proteomic profiling of TPD.
HostingRepository	iProX
AnnounceDate	2024-03-06
AnnouncementXML	Submission_2024-03-06_17:52:44.637.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuying Suo
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-06 17:52:11	ID requested
⏵ 1	2024-03-06 17:52:45	announced
2	2024-03-12 22:54:02	announced	2024-03-13: Update information.

Dataset with its publication pending

submitter keyword: Targeted protein degradation, Mass spectrometry-based Proteomics, Data-independent Acquisition, Drug sensitivity

Hu Zhou
contact affiliation	Shanghai Insititute of Materia Medica
contact email	zhouhu@simm.ac.cn
lab head
Yuying Suo
contact affiliation	Shanghai Insititute of Materia Medica
contact email	s20-suoyuying@simm.ac.cn
dataset submitter

iProX dataset URI