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PXD049141-1

PXD049141 is an original dataset announced via ProteomeXchange.

Dataset Summary
Title[HPP-2.0] Dynamic phosphotyrosine-dependent signaling profiling in living cells by two-dimensional proximity proteomics
DescriptionTyrosine phosphorylation (pTyr)-dependent signaling pathways play vital role in various biological processes, which are spatiotemporally assembled and dynamically regulated on minute-scale by pTyr in living cells. Studying these pTyr-mediated signaling complexes is therefore challenging due to the highly dynamic nature of the protein complexes and the low abundance of pTyr. In this study, we adopted minute-resolution APEX2-based proximity labeling (PL) in living cells and Src SH2 superbinder-based pTyr peptide enrichment for simultaneously profiling these protein complexes and associated pTyr sites from the same affinity-purified sample. Upon different time-course of EGF stimulation of the living cells stably expressing APEX2-FLAG-GRB2, we constructed two-dimensional time-course curves for both interactome and tyrosine phosphoproteome.
HostingRepositoryiProX
AnnounceDate2024-02-03
AnnouncementXMLSubmission_2024-02-03_19:46:35.807.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRuijun Tian
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-02-03 19:45:20ID requested
12024-02-03 19:46:36announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: phosphotyrosine, living cells, proximity proteomics
Contact List
Ruijun Tian
contact affiliationSouthern University of Science and Technology
contact emailtianrj@sustech.edu.cn
lab head
Ruijun Tian
contact affiliationSouthern University of Science and Technology
contact email13667356941@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI