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PXD048855-1

PXD048855 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUnravelling Intracellular Protein Corona Components of Nanoplas-tics via Photocatalytic Protein Proximity Labeling
DescriptionBioaccumulation of nanoplastic particles draws increasing attention regarding environmental sustainability and biosafety. How nanoplastic particles interact with cellular milieu still remains elusive. Herein, we exemplify a general approach to profile the composition of “protein corona” interacting with nanoparticles via the photocatalytic protein proximity labeling method. To enable photocatalytic proximity labeling of proteome interacting with particles, iodine substituted BODIPY (I-BODIPY) is selected as the photosensitizer and covalently conjugated onto amino-polystyrene nanoparticle as a model system. Next, selec-tive proximity labeling of interacting proteins is demonstrated using I-BODIPY labeled nanoplastic particles in both E. coli lysate and live AML 12 cells. Mechanistic studies reveal the covalent modifications of proteins by amino-alkyne substrate is conducted via reactive oxygen species (ROS) photosensitization pathway. Further proteomic analysis uncovers that mitochon-dria-related proteins are intensively involved in protein corona, indicating substantial interactions between nanoplastic particles and mitochondria. In addition, proteostasis network components are also identified accompanied with consequent cellular pro-teome aggregation confirmed by fluorescence imaging. Together, this work exemplifies a general strategy to interrogate the composition of protein corona of nanomaterials by grafting them photo-oxidation property to enable photocatalytic protein proximity labeling function.
HostingRepositoryiProX
AnnounceDate2024-01-24
AnnouncementXMLSubmission_2024-01-24_18:39:12.843.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZhenduo Zhang
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-24 18:38:49ID requested
12024-01-24 18:39:13announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: protein corona, nanoplastic, proximity labeling, proteomics, mitochondria.
Contact List
Zhenduo Zhang
contact affiliationDalian Institute of Chemical Physics
contact email17615003187@163.com
lab head
Zhenduo Zhang
contact affiliationDalian Institute of Chemical Physics
contact email17615003187@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI