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PXD048553-1

PXD048553 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA new technology for isolating organellar membranes provides fingerprints of lipid bilayer stress
DescriptionBiological membranes have a stunning ability to adapt their composition in response to physiological stress and metabolic challenges. Little is known how such perturbations affect individual organelles in eukaryotic cells. Pioneering work provided insights into the subcellular distribution of lipids in the yeast Saccharomyces cerevisiae, but the composition of the endoplasmic reticulum (ER) membrane, which also crucially regulates lipid metabolism and the unfolded protein response, remained insufficiently characterized. Here we describe a method for purifying organelle membranes from yeast, MemPrep. We demonstrate the purity of our ER membrane preparations by proteomics and document the general utility of MemPrep by isolating vacuolar membranes. Quantitative lipidomics establishes the lipid composition of the ER and the vacuolar membrane. Our findings provide a baseline for studying membrane protein biogenesis and have important implications for understanding the role of lipids in regulating the unfolded protein response (UPR). The combined preparative and analytical MemPrep approach uncovers a dynamic remodeling of ER membranes in stressed cells and establishes distinct molecular fingerprints of lipid bilayer stress.
HostingRepositoryPRIDE
AnnounceDate2024-03-05
AnnouncementXMLSubmission_2024-03-05_01:54:53.651.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFrank Stein
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-15 03:08:01ID requested
12024-03-05 01:54:54announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: organelle isolation, proteomics, UPR, lipid bilayer stress,lipidomics, membrane
Contact List
Robert Ernst
contact affiliationSaarland University Preclinical Center for Molecular Signalling (PZMS) Institute of Medical Biochemistry and Molecular Biology
contact emailrobert.ernst@uks.eu
lab head
Frank Stein
contact affiliationEMBL
contact emailfrank.stein@embl.de
dataset submitter
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Dataset FTP location
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