<<< Full experiment listing

PXD047810

PXD047810 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Phosphoproteomics profiling of sorafenib-resistant HCC patient-derived xenografts reveals potential therapeutic strategies
Description	Hepatocellular carcinoma (HCC) is the most common form of primary liver cancer with poor prognosis. However, effective treatment options for advanced HCC are limited. Sorafenib, a first-line treatment for advanced HCC, has shown limited clinical benefits due to the onset of drug resistance. Thus, it is imperative to comprehend the mechanisms underlying sorafenib resistance and explore novel strategies to overcome or delay it. Here, we established HCC patient-derived xenograft (PDX) models with acquired resistance to sorafenib and performed comprehensive proteomic and phosphoproteomic analyses on these models. This investigation led to the identification of 9,366 proteins and 20,127 unique phosphosites. The active cell cycle pathway, along with the active cyclin-dependent kinase CDK1 and DNA-dependent protein kinase PRKDC, was identified through KEGG pathway enrichment and kinase substrate enrichment analyses. Upon investigating the potential of combining sorafenib with putative kinase inhibitors, we found that the combination displays synergistic anti-proliferative effects in both the sorafenib-resistant liver cancer cell line and PDX models, thus providing a proof-of-concept for phosphoproteomic-guided design of precision medicine.
HostingRepository	iProX
AnnounceDate	2023-12-14
AnnouncementXML	Submission_2023-12-13_19:12:39.406.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Feng Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-13 19:12:27	ID requested
⏵ 1	2023-12-13 19:12:39	announced

Publication List

Dataset with its publication pending

Keyword List

submitter keyword: Phosphorylation

HCC

Contact List

Haojie Lu
contact affiliation	Institutes of Biomedical Sciences, Fudan University
contact email	luhaojie@fudan.edu.cn
lab head
Feng Li
contact affiliation	Institutes of Biomedical Sciences, Fudan University
contact email	lifengsci@163.com
dataset submitter

Full Dataset Link List

iProX dataset URI