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PXD047719-1

PXD047719 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNative Top-Down Mass Spectrometry for Characterizing Sarcomeric Proteins Directly from Cardiac Tissue Lysate
DescriptionNative top-down mass spectrometry is a powerful structural biology tool that can localize post-translational modifications, explore ligand-binding interactions, and elucidate the three-dimensional structure of proteins and protein complexes in the gas-phase. Fourier-transform ion cyclotron resonance-MS offers distinct capabilities for nTDMS, owing to its ultra-high resolving power, mass accuracy, and robust fragmentation techniques. Previous nTDMS studies using FTICR have mainly applied to over-expressed recombinant proteins and protein complexes. Here, we report the first nTDMS study that directly analyzes human heart tissue lysate by direct infusion FTICR-MS without prior chromatographic separation strategies. We have achieved comprehensive nTDMS characterization of cardiac contractile proteins that play critical roles in heart contraction and relaxation. Specifically, our results reveal structural insights into ventricular myosin light chain 2, ventricular myosin light chain 1, and alpha-tropomyosin in the sarcomere, the basic contractile unit of cardiac muscle. Furthermore, we localize the calcium binding domain in MLC-2v. In summary, our nTDMS platform extends the application of FTICR-MS to directly characterize the structure, PTMs, and metal-binding of endogenous proteins from heart tissue lysate without prior separation methods.
HostingRepositoryMassIVE
AnnounceDate2024-03-13
AnnouncementXMLSubmission_2024-03-13_07:18:11.946.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEmily Chapman
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListTrimethyl; Phospho; Acetyl
Instrumentinstrument model
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-11 08:51:07ID requested
12024-03-13 07:18:12announced
Publication List
no publication
Keyword List
submitter keyword: Native top-down, cardiac, FTICR, endogenous, structural biology
Contact List
Ying Ge
contact affiliationUniversity of Wisconsin - Madison
contact emailying.ge@wisc.edu
lab head
Emily Chapman
contact affiliationUniversity of Wisconsin - Madison
contact emaileachapman2@wisc.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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