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PXD047669-1

PXD047669 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMulti-omics analysis of aggregative multicellularity
DescriptionThe extent to which mRNA and protein levels correlate is still not fully known, especially during development, when cells undergo a major transition in gene expression. One organism with particular development is Dictyostelium discoideum, during which it transitions from free-living unicellular to multicellular. Previously the transcriptome has been thoroughly studied during multicellular development, however the proteome and its correlation to the transcriptome during this transition is not fully understood. In this study, for the first time, paired transcriptomics and proteomics was performed in a time series during aggregative multicellularity. From the analysis, the majority of transcripts were identified as differentially expressed, and we could quantify roughly a third of the proteome during early multicellular development. The proteome and transcriptome correlate relatively highly during steady-state, however this decreases as soon as multicellular aggregation is initiated. Correlation is particularly low at the gene-level during development. We find that dynamically regulated mRNA often leads to linear up- or downregulation of the protein, and that there is a time lag of approximately 2 to 4 hours between mRNA and protein.
HostingRepositoryMassIVE
AnnounceDate2024-03-08
AnnouncementXMLSubmission_2024-03-08_05:49:27.818.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterBart Edelbroek
SpeciesList scientific name: Dictyostelium discoideum; NCBI TaxID: 44689;
ModificationListOxidation; GG; Carbamidomethyl; Acetyl
InstrumentQ Exactive GC Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-09 12:05:04ID requested
12024-03-08 05:49:28announced
Publication List
no publication
Keyword List
submitter keyword: Multi-omics, Transcriptomics, Proteomics, mRNA-protein correlation, Aggregative multicellularity, Amoebozoa, Dictyostelium discoideum
Contact List
Fredrik Soderbom
contact affiliationUppsala University
contact emailfredrik.soderbom@icm.uu.se
lab head
Bart Edelbroek
contact affiliationUppsala University
contact emailbart.edelbroek@gmail.com
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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