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PXD047668-1

PXD047668 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCSB promotes transcription-coupled repair of DNA-protein crosslinks
DescriptionPxP-MS (Purification of x-linked Proteins coupled to Mass Spectrometry) was used to assess the role of CSB in DNA-protein crosslink repair. The CSB protein is a sensor that can detect stalled RNA polymerases at sites of DNA damage, thereby triggering transcription-coupled repair mechanisms. DPCs were induced in WT and CSB knock-out RPE1 cells using a pulse of formaldehyde. To identify crosslinked proteins that specifically require CSB for repair, DNA-protein crosslinks were isolated from cells using PxP either directly after formaldehyde exposure or following a chase in drug-free media and identified by mass spectrometry.
HostingRepositoryPRIDE
AnnounceDate2024-02-15
AnnouncementXMLSubmission_2024-02-15_09:08:28.156.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFatih Aygenli
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-09 06:34:03ID requested
12024-02-15 09:08:28announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Cockayne syndrome, formaldehyde, DNA damage repair, DNA-protein crosslinks, CSB
Contact List
Charo Robles
contact affiliationSystem Chronobiology Institute for Medical Psychology Ludwig-Maximilians Universitat Muenchen Germany (lab head)
contact emailcharo.robles@med.uni-muenchen.de
lab head
Fatih Aygenli
contact affiliationLMU - Muenchen
contact emailfatih.aygenli@med.uni-muenchen.de
dataset submitter
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Dataset FTP location
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Repository Record List
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