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PXD047493-1

PXD047493 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHydrogen sulfide mediated persulfidation regulates homocysteine metabolism and enhances ferroptosis in non-small-cell lung cancer
DescriptionFerroptosis, a novel form of regulated cell death triggered by the iron-dependent peroxidation of phospholipids, offers promising penitential for cancer therapy. Hydrogen sulfide (H2S), an endogenous metabolite of the transsulfuration pathway, has been implicated in ferroptosis. However, the precise regulatory mechanisms remain elusive. In this study, we systematically investigated the role of exogenous hydrogen sulfide in ferroptosis regulation in non-small-cell lung cancer (NSCLC). We demonstrated that H2S sensitizes NSCLC to ferroptosis both in vitro and in vivo. Mechanistically, hydrogen sulfide persulfurates the 195th cysteine residue of S-adenosyl homocysteine hydrolase (SAHH), thus impeding the binding of SAHH to its substrate S-adenosyl homocysteine (SAH). Consequently, this inhibition reduces SAHH activity, leading to a decreased homocysteine level. This reduction in homocysteine subsequently results in diminished levels of cysteine and glutathione, particularly under conditions of cystine depletion, which collectively potentiate the occurrence of ferroptosis. Together, our findings unveil H2S as pivotal regulator for homocysteine metabolism, increasing the sensitivity of NSCLC to ferroptosis. Importantly, these findings highlight its potential therapeutic value for enhancing ferroptosis-based cancer therapies for NCSCLC.
HostingRepositoryiProX
AnnounceDate2023-12-04
AnnouncementXMLSubmission_2023-12-21_22:48:38.470.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHualei Zheng
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF Pro; QTRAP 5500
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-03 23:57:49ID requested
12023-12-21 22:48:38announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Ferroptosis,Transsulfuration pathway,Hydrogen sulfide (H2S),Persulfidation,S-adenosyl homocysteine hydrolase (SAHH)
Contact List
Yongyou Zhang
contact affiliationXiamen University
contact emailyongyouzhang@xmu.edu.cn
lab head
Hualei Zheng
contact affiliationXiamen University
contact emailhlzheng12300@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI