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PXD047419-1

PXD047419 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDecrypting lysine deacetylase inhibitor action and protein modifications by dose-resolved proteomics
DescriptionLysine deacetylase inhibitors (KDACis) are approved drugs for cutaneous T-cell lymphoma (CTCL) and multiple myeloma. The drugs lead to increasing acetylation levels of histones and other proteins, altered gene expression, and cell death. To characterize the mechanisms of action of these drugs in more detail, we systematically measured dose-dependent drug-induced changes in protein expression, acetylation, and phosphorylation in response to 21 clinical and pre-clinical KDACis. The resulting 872,000 dose-response curves revealed, for instance, poor selectivity of HDAC1, 2, 3, and 6 inhibitors in cells, strong cross-talk between acetylation and phosphorylation pathways, localization of most drug-responsive acetylation sites to intrinsically disordered regions (IDRs), an underappreciated role of acetylation in protein structure and a shift in EP300 protein abundance between the cytoplasm and the nucleus. This comprehensive dataset serves as a resource for the investigation of the molecular mechanisms underlying KDACi action in cells and can be explored online in ProteomicsDB.
HostingRepositoryPanoramaPublic
AnnounceDate2024-04-21
AnnouncementXMLSubmission_2024-04-21_22:42:29.356.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMiriam Abele
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListAcetyl; Amidated; Oxidation
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-30 14:28:38ID requested
12024-04-21 22:42:29announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Chemical proteomics, acetylation, phosphorylation, lysine deacetylase inhibitors, HDACs, proteomic pharmacology
Contact List
Bernhard Kuster
contact affiliationChair of Proteomics and Bioanalytics, TUM School of Life Sciences, Technical University of Munich, Freising, Germany
contact emailkuster@tum.de
lab head
Miriam Abele
contact affiliationChair of Proteomics and Bioanalytics, TUM School of Life Sciences, Technical University of Munich, Freising, Germany
contact emailm.abele@tum.de
dataset submitter
Full Dataset Link List
Panorama Public dataset URI