PXD047361

PXD047361 is an original dataset announced via ProteomeXchange.

Title	Proteomic response of HepG2 cells with SC-43 treatment
Description	HepG2 cells were seeded in six-well plates at a density of 1×106 cells per well. The cells were divided into the control group and the SC-43 group. The cells were exposed to a DMEM culture medium containing DMSO in the control group. The SC-43 group with the added SC-43 (4-hour node CC50 concentration) was then incubated for another 2h. Subsequently, the cells were rinsed twice with pre-cooled PBS and harvested with cell lysis buffer. The lysis buffer contained protease inhibitors and phosphatase inhibitors. The lysates were centrifuged at 12000 rpm for 10 minutes, 4℃, and the resulting supernatants were gathered as protein specimens. The protein reduction, alkylation and tryptic digestion were followed as filter-aided sample preparation (FASP). The digestion products were analyzed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), followed by label-free quantitative analysis. The MS experiment was performed on a Q Exactive Plus mass spectrometer.
HostingRepository	iProX
AnnounceDate	2023-11-29
AnnouncementXML	Submission_2023-11-28_22:58:32.900.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ting Mao
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-28 22:58:11	ID requested
⏵ 1	2023-11-28 22:58:33	announced

Dataset with its publication pending

submitter keyword: SC-43, HepG2 cells

Peiyu Li
contact affiliation	Nanshan Hospital
contact email	peiyuli12@fudan.edu.cn
lab head
Ting Mao
contact affiliation	Beijing University of Chinese Medicine
contact email	1085781829@qq.com
dataset submitter

iProX dataset URI