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PXD047042-1

PXD047042 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIn vivo mapping of protein-protein interactions of schizophrenia risk factors generates an interconnected disease network.
DescriptionGenetic analyses of Schizophrenia (SCZ) patients have identified thousands of risk factors. In silico protein-protein interaction (PPI) network analysis has provided strong evidence that disrupted PPI networks underlie SCZ pathogenesis. In this study, we performed in vivo PPI analysis of several SCZ risk factors in the rodent brain. Using endogenous antibody immunoprecipitations coupled to mass spectrometry (MS) analysis, we constructed a SCZ network comprising 1612 unique PPI with a 5% FDR. Over 90% of the PPI were novel, reflecting the lack of previous PPI MS studies in brain tissue. Our SCZ PPI network was enriched with known SCZ risk factors, which supports the hypothesis that an accumulation of disturbances in selected PPI networks underlies SCZ. We used Stable Isotope Labeling in Mammals (SILAM) to quantitate phencyclidine (PCP) perturbations in the SCZ network and found that PCP weakened most PPI but also led to some enhanced or new PPI. These findings demonstrate that quantitating PPI in perturbed biological states can reveal alterations to network biology.
HostingRepositoryMassIVE
AnnounceDate2023-12-12
AnnouncementXMLSubmission_2023-12-12_11:13:35.945.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDaniel McClatchy
SpeciesList scientific name: Rattus norvegicus; common name: Norway rat; NCBI TaxID: 10116;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-17 11:59:09ID requested
12023-12-12 11:13:36announced
Publication List
no publication
Keyword List
submitter keyword: schizophrenia, SILAM, protein-protein interactions, network analysis, phencyclidine
Contact List
John R. Yates III
contact affiliationThe Scripps Research Institute
contact emailjyates@scripps.edu
lab head
Daniel McClatchy
contact affiliationTSRI
contact emaildmcclat@scripps.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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