PXD047042
PXD047042 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | In vivo mapping of protein-protein interactions of schizophrenia risk factors generates an interconnected disease network. |
| Description | Genetic analyses of Schizophrenia (SCZ) patients have identified thousands of risk factors. In silico protein-protein interaction (PPI) network analysis has provided strong evidence that disrupted PPI networks underlie SCZ pathogenesis. In this study, we performed in vivo PPI analysis of several SCZ risk factors in the rodent brain. Using endogenous antibody immunoprecipitations coupled to mass spectrometry (MS) analysis, we constructed a SCZ network comprising 1612 unique PPI with a 5% FDR. Over 90% of the PPI were novel, reflecting the lack of previous PPI MS studies in brain tissue. Our SCZ PPI network was enriched with known SCZ risk factors, which supports the hypothesis that an accumulation of disturbances in selected PPI networks underlies SCZ. We used Stable Isotope Labeling in Mammals (SILAM) to quantitate phencyclidine (PCP) perturbations in the SCZ network and found that PCP weakened most PPI but also led to some enhanced or new PPI. These findings demonstrate that quantitating PPI in perturbed biological states can reveal alterations to network biology. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-12-12 |
| AnnouncementXML | Submission_2023-12-12_11:13:35.945.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Daniel McClatchy |
| SpeciesList | scientific name: Rattus norvegicus; common name: Norway rat; NCBI TaxID: 10116; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-11-17 11:59:09 | ID requested | |
| ⏵ 1 | 2023-12-12 11:13:36 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: schizophrenia, SILAM, protein-protein interactions, network analysis, phencyclidine |
Contact List
| John R. Yates III | |
|---|---|
| contact affiliation | The Scripps Research Institute |
| contact email | jyates@scripps.edu |
| lab head | |
| Daniel McClatchy | |
| contact affiliation | TSRI |
| contact email | dmcclat@scripps.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000093425/ |
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