PXD046374-1
PXD046374 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | The phosphorylation sites in GLI1 analyzed by mass-spec methodology |
Description | The mass spectrometry analysis was performed by Micrometer Biotech Company. Endogenous human GLI1 from Daoy cells was enriched by co-immunoprecipitation with anti-Gli1 and purified by SDS-PAGE. The gel was then stained with Coomassie brilliant blue, and the corresponding visualized bands were excised, destained with ammonium bicarbonate buffer and dehydrated in 75% acetonitrile. After rehydration with ammonium bicarbonate, trypsin digestion was performed, followed by peptide extraction with acetonitrile, drying, and mass spectrometry |
HostingRepository | iProX |
AnnounceDate | 2023-10-25 |
AnnouncementXML | Submission_2023-10-24_23:01:35.580.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jirong Wang |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-10-24 23:01:18 | ID requested | |
⏵ 1 | 2023-10-24 23:01:35 | announced | |
2 | 2024-02-26 20:07:38 | announced | 2024-02-27: Update information. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: phosphorylation sites, GLI1 |
Contact List
Ximei Wu | |
---|---|
contact affiliation | Zhejiang University |
contact email | xiwu@zju.edu.cn |
lab head | |
Jirong Wang | |
contact affiliation | Zhejiang University |
contact email | wangjr@zju.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |