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PXD046374-1

PXD046374 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe phosphorylation sites in GLI1 analyzed by mass-spec methodology
DescriptionThe mass spectrometry analysis was performed by Micrometer Biotech Company. Endogenous human GLI1 from Daoy cells was enriched by co-immunoprecipitation with anti-Gli1 and purified by SDS-PAGE. The gel was then stained with Coomassie brilliant blue, and the corresponding visualized bands were excised, destained with ammonium bicarbonate buffer and dehydrated in 75% acetonitrile. After rehydration with ammonium bicarbonate, trypsin digestion was performed, followed by peptide extraction with acetonitrile, drying, and mass spectrometry
HostingRepositoryiProX
AnnounceDate2023-10-25
AnnouncementXMLSubmission_2023-10-24_23:01:35.580.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJirong Wang
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-24 23:01:18ID requested
12023-10-24 23:01:35announced
22024-02-26 20:07:38announced2024-02-27: Update information.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: phosphorylation sites, GLI1
Contact List
Ximei Wu
contact affiliationZhejiang University
contact emailxiwu@zju.edu.cn
lab head
Jirong Wang
contact affiliationZhejiang University
contact emailwangjr@zju.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI