PXD046374

PXD046374 is an original dataset announced via ProteomeXchange.

Title	The phosphorylation sites in GLI1 analyzed by mass-spec methodology
Description	The mass spectrometry analysis was performed by Micrometer Biotech Company. Endogenous human GLI1 from Daoy cells was enriched by co-immunoprecipitation with anti-Gli1 and purified by SDS-PAGE. The gel was then stained with Coomassie brilliant blue, and the corresponding visualized bands were excised, destained with ammonium bicarbonate buffer and dehydrated in 75% acetonitrile. After rehydration with ammonium bicarbonate, trypsin digestion was performed, followed by peptide extraction with acetonitrile, drying, and mass spectrometry
HostingRepository	iProX
AnnounceDate	2023-10-25
AnnouncementXML	Submission_2024-02-26_20:07:37.735.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jirong Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-24 23:01:18	ID requested
1	2023-10-24 23:01:35	announced
⏵ 2	2024-02-26 20:07:38	announced	2024-02-27: Update information.

Zeng LH, Tang C, Yao M, He Q, Qv M, Ren Q, Xu Y, Shen T, Gu W, Xu C, Zou C, Ji X, Wu X, Wang J, Phosphorylation of human glioma-associated oncogene 1 on Ser937 regulates Sonic Hedgehog signaling in medulloblastoma. Nat Commun, 15(1):987(2024) [pubmed]

submitter keyword: phosphorylation sites, GLI1

Ximei Wu
contact affiliation	Zhejiang University
contact email	xiwu@zju.edu.cn
lab head
Jirong Wang
contact affiliation	Zhejiang University
contact email	wangjr@zju.edu.cn
dataset submitter

iProX dataset URI