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PXD046297-1

PXD046297 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBIN2 Inhibition Suppress Cancer Progression and Protect Ovarian function through Downregulating HDAC1 Phosphorylation
DescriptionMalignant tumors, or cancers, are increasingly menacing people's life and health. For your female cancer patient, the ideal therapy should achieve dual purpose: suppress tumor progression meanwhile protect ovarian function. We previously showed that activated (phosphorylated) BIN2 in mouse ovaries regulates primordial follicle activation and oocyte quality through p-RPS6, and in this study, we found that BIN2 knockout or inhibition of BIN2 phosphorylation by BPP could suppress the genesis and progression of ovarian cancer. However, in human female ovarian cancer tissues, we didn’t see the increment of p-RPS6 although we observed a significant raise of p-BIN2. From this discrepancy between normal ovaries and ovarian cancer tissue, we guessed that p-BIN2 has other targets that are more important in ovarian cancer progression. Through mass spec identification, we found that only the constitutively active form of BIN2 (T423D & S424D) baits HDAC1, indicating that HDAC1 is a more important target of BIN2 in ovarian cancer. Next, we did saw Bin2 knockout or inhibition significantly decreased p-HDAC1 (S421) meanwhile increased H3K27ac. Moreover, chip seq showed that BIN2 inhibition significantly increased the binding of H3K27ac to multiple tumor suppressors. Besides, BIN2 knockout or inhibition could meanwhile protect ovarian function in mice with chemical carcinogen-induced in-situ ovarian cancers or with ovarian cancer cell transplantation. This study suggested that BIN2 inhibition could both suppress ovarian tumorigenesis and protect ovarian fertility, but through distinct mechanisms.
HostingRepositoryiProX
AnnounceDate2023-10-20
AnnouncementXMLSubmission_2023-10-22_19:20:43.162.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterCongrong Li
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-22 19:20:26ID requested
12023-10-22 19:20:43announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: BIN2,tumor progression, HDAC1, phosphorylation
Contact List
Dong Zhang
contact affiliationDepartment of Gynaecology and Obstetrics, the First Affiliated Hospital of Anhui Medical University
contact emaildong.ray.zhang@ahmu.edu.cn
lab head
Congrong Li
contact affiliationNanjing Medical university
contact email986162264@qq.com
dataset submitter
Full Dataset Link List
iProX dataset URI