PXD046020

PXD046020 is an original dataset announced via ProteomeXchange.

Title	Chromatin Isolation by RNA Purification (ChIRP) assays followed by LC-MS in DCRT-KO cell lines
Description	To explore the molecular mechanism of DCRT in DCM, Chromatin Isolation by RNA Purification (ChIRP) assays followed by Liquid Chromatography Mass Spectrometry (LC-MS) analyses were conducted. Each probe was designed using the web-based designer provided by Sangon Biotech, biotin-TEG-labeled separated into odd and even groups. Probes that targeted LacZ were utilized as a safeguard. For each sample, 4 × 10^7 WT or DCRT-KO AC16 cells were used and crosslinked in 1% glutaraldehyde at room temperature for 10 min, and quenched with 125 mM glycine for 5 min. Cleared lysates were hybridized with probes in 37 °C in a hybridization oven for 4 h. After washes and elution, proteins were purified and LC-MS
HostingRepository	iProX
AnnounceDate	2023-10-10
AnnouncementXML	Submission_2023-10-10_00:50:47.496.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hengzhi Du
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-10 00:49:52	ID requested
⏵ 1	2023-10-10 00:50:47	announced

Dataset with its publication pending

submitter keyword: ChIRP, DCRT, LC-MS

Chen Chen
contact affiliation	Huazhong University of Science and Technology Tongji Hospital, Tongji Medical College
contact email	chenchen@tjh.tjmu.edu.cn
lab head
Hengzhi Du
contact affiliation	Huazhong University of Science and Technology Tongji Hospital, Tongji Medical College
contact email	duhengzhi_tongji@163.com
dataset submitter

iProX dataset URI