To explore the molecular mechanism of DCRT in DCM, Chromatin Isolation by RNA Purification (ChIRP) assays followed by Liquid Chromatography Mass Spectrometry (LC-MS) analyses were conducted. Each probe was designed using the web-based designer provided by Sangon Biotech, biotin-TEG-labeled separated into odd and even groups. Probes that targeted LacZ were utilized as a safeguard. For each sample, 4 × 10^7 WT or DCRT-KO AC16 cells were used and crosslinked in 1% glutaraldehyde at room temperature for 10 min, and quenched with 125 mM glycine for 5 min. Cleared lysates were hybridized with probes in 37 °C in a hybridization oven for 4 h. After washes and elution, proteins were purified and LC-MS