PXD045987 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometry analysis of chromosomal passenger complex proteins and kinetochore proteins in Trypanosoma brucei |
Description | We performed chemical crosslinking mass spectrometry of native protein complexes purified from Trypanosoma brucei procyclic cells to obtain protein-protein interaction information for the chromosomal passenger complex (CPC) and kinetochore complex. Crosslinks among CPC subunits were detected using YFP-AUK1, while those among KKT8 complex subunits were detected in the YFP-KKIP1 sample. We also performed immunoprecipitation and mass spectrometry of CPC subunits (full-length proteins and fragments) to identify co-purifying proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2023-10-13 |
AnnouncementXML | Submission_2023-10-13_00:40:56.661.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | BungoAkiyoshi |
SpeciesList | scientific name: Trypanosoma brucei; NCBI TaxID: 5691; |
ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-10-09 05:09:36 | ID requested | |
⏵ 1 | 2023-10-13 00:40:57 | announced | |
2 | 2023-11-14 08:25:01 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Kinetochore, crosslinking mass spectrometry |
Contact List
BungoAkiyoshi |
contact affiliation | University of Edinburgh |
contact email | bungo.akiyoshi@ed.ac.uk |
lab head | |
BungoAkiyoshi |
contact affiliation | University of Edinburgh |
contact email | bungo.akiyoshi@ed.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045987
- Label: PRIDE project
- Name: Mass spectrometry analysis of chromosomal passenger complex proteins and kinetochore proteins in Trypanosoma brucei