PXD045987

PXD045987 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry analysis of chromosomal passenger complex proteins and kinetochore proteins in Trypanosoma brucei
Description	We performed chemical crosslinking mass spectrometry of native protein complexes purified from Trypanosoma brucei procyclic cells to obtain protein-protein interaction information for the chromosomal passenger complex (CPC) and kinetochore complex. Crosslinks among CPC subunits were detected using YFP-AUK1, while those among KKT8 complex subunits were detected in the YFP-KKIP1 sample. We also performed immunoprecipitation and mass spectrometry of CPC subunits (full-length proteins and fragments) to identify co-purifying proteins.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:25:00.879.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bungo Akiyoshi
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-09 05:09:36	ID requested
1	2023-10-13 00:40:57	announced
⏵ 2	2023-11-14 08:25:01	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Kinetochore, crosslinking mass spectrometry

Bungo Akiyoshi
contact affiliation	University of Edinburgh
contact email	bungo.akiyoshi@ed.ac.uk
lab head
Bungo Akiyoshi
contact affiliation	University of Edinburgh
contact email	bungo.akiyoshi@ed.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045987
     1. Label: PRIDE project
     2. Name: Mass spectrometry analysis of chromosomal passenger complex proteins and kinetochore proteins in Trypanosoma brucei