PXD045843

PXD045843 is an original dataset announced via ProteomeXchange.

Title	Allele-specific iChIP-SILAC analysis to identify proteins binding to the p16INK4A gene
Description	We used insertional ChIP (iChIP) based approach to identify proteins that bind to the p16INK4A gene in the human cell line HCT116. To perform iChIP, we inserted an 8-repeat of the LexA-binding element (LexA-BE) in the p16INK4A promoter region in HCT116. The DNA-binding domain and dimerization domain of the LexA protein fused with the 3xFLAG tag and a nuclear localization signal (NLS) was expressed in the LexA-BE-knock-in cells (#109-2 and #113-5). The resultant cells (#109-2-1 and #113-5-1) were subjected to stable isotope labeling with amino acids in cell culture (SILAC), immunoprecipitated with an anti-FLAG antibody, and subjected to LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2024-04-18
AnnouncementXML	Submission_2024-04-18_02:37:25.175.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Toshitsugu Fujita
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; 6x(13)C labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-01 18:33:22	ID requested
⏵ 1	2024-04-18 02:37:25	announced

10.1002/1873-3468.14878;

submitter keyword: Human, SILAC, iChIP

Hodaka Fujii
contact affiliation	Hirosaki University Graduate School of Medicine, Department of Biochemistry and Genome Biology, JAPAN
contact email	hodaka@hirosaki-u.ac.jp
lab head
Toshitsugu Fujita
contact affiliation	Hirosaki University Graduate School of Medicine
contact email	toshitsugu.fujita@hirosaki-u.ac.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045843
     1. Label: PRIDE project
     2. Name: Allele-specific iChIP-SILAC analysis to identify proteins binding to the p16INK4A gene