We used insertional ChIP (iChIP) based approach to identify proteins that bind to the p16INK4A gene in the human cell line HCT116. To perform iChIP, we inserted an 8-repeat of the LexA-binding element (LexA-BE) in the p16INK4A promoter region in HCT116. The DNA-binding domain and dimerization domain of the LexA protein fused with the 3xFLAG tag and a nuclear localization signal (NLS) was expressed in the LexA-BE-knock-in cells (#109-2 and #113-5). The resultant cells (#109-2-1 and #113-5-1) were subjected to stable isotope labeling with amino acids in cell culture (SILAC), immunoprecipitated with an anti-FLAG antibody, and subjected to LC-MS/MS analysis.