PXD045723 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Interplay between cultured human osteoblastic and skeletal muscle cells: Effects of conditioned media on glucose and fatty acid metabolism |
Description | The interplay between skeletal muscle and bone is primarily mechanical, however, biochemical crosstalk by secreted mediators has recently gained increased attention. The aim of this study was to investigate metabolic effects of conditioned medium from osteoblasts (OB-CM) on myotubes and vice versa. Human skeletal muscle cells incubated with OB-CM showed increased glucose uptake and oxidation, and expression of the glucose transporter (GLUT) 1, while fatty acid uptake and oxidation, and expression of the fatty acid transporter CD36 were decreased. This was supported by proteomic analysis where expression of proteins involved in glucose uptake, glycolytic pathways and TCA cycle were enhanced, and expression of several proteins involved in fatty acid metabolism were reduced. Similar effects on energy metabolism were observed in human bone marrow stromal cells differentiated to osteoblastic cells incubated with conditioned medium from myotubes (SKM-CM), with increased glucose uptake and reduced oleic acid uptake. Proteomic analyses of the two conditioned media revealed many common proteins. Thus, our data may indicate a shift in fuel preference from fatty acid to glucose metabolism in both cell types, induced by conditioned media from the opposite cell type, possibly indicating a more general pattern in communication between these tissues. |
HostingRepository | PRIDE |
AnnounceDate | 2024-01-26 |
AnnouncementXML | Submission_2024-01-26_07:49:33.980.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sachin Singh |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; timsTOF fleX |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-26 23:43:37 | ID requested | |
⏵ 1 | 2024-01-26 07:49:34 | announced | |
Publication List
Lunde NN, Osoble NMM, Fernandez AD, Antobreh AS, Jafari A, Singh S, Nyman TA, Rustan AC, Solberg R, Thoresen GH, Interplay between Cultured Human Osteoblastic and Skeletal Muscle Cells: Effects of Conditioned Media on Glucose and Fatty Acid Metabolism. Biomedicines, 11(11):(2023) [pubmed] |
10.3390/biomedicines11112908; |
Keyword List
submitter keyword: Energy metabolism |
Myokines |
Osteoblastic cells |
Osteokines |
Primary human myotubes; |
Contact List
G. Hege Thoresen |
contact affiliation | Section for Pharmacology and Pharmaceutical Biosciences, Department of Pharmacy, University of Oslo, |
contact email | hege.thoresen@farmasi.uio.no |
lab head | |
Sachin Singh |
contact affiliation | Proteomics core facility, Olso University hospital, Norway |
contact email | sachin.kumar.singh@rr-research.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045723
- Label: PRIDE project
- Name: Interplay between cultured human osteoblastic and skeletal muscle cells: Effects of conditioned media on glucose and fatty acid metabolism