PXD045723

PXD045723 is an original dataset announced via ProteomeXchange.

Title	Interplay between cultured human osteoblastic and skeletal muscle cells: Effects of conditioned media on glucose and fatty acid metabolism
Description	The interplay between skeletal muscle and bone is primarily mechanical, however, biochemical crosstalk by secreted mediators has recently gained increased attention. The aim of this study was to investigate metabolic effects of conditioned medium from osteoblasts (OB-CM) on myotubes and vice versa. Human skeletal muscle cells incubated with OB-CM showed increased glucose uptake and oxidation, and expression of the glucose transporter (GLUT) 1, while fatty acid uptake and oxidation, and expression of the fatty acid transporter CD36 were decreased. This was supported by proteomic analysis where expression of proteins involved in glucose uptake, glycolytic pathways and TCA cycle were enhanced, and expression of several proteins involved in fatty acid metabolism were reduced. Similar effects on energy metabolism were observed in human bone marrow stromal cells differentiated to osteoblastic cells incubated with conditioned medium from myotubes (SKM-CM), with increased glucose uptake and reduced oleic acid uptake. Proteomic analyses of the two conditioned media revealed many common proteins. Thus, our data may indicate a shift in fuel preference from fatty acid to glucose metabolism in both cell types, induced by conditioned media from the opposite cell type, possibly indicating a more general pattern in communication between these tissues.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_07:49:33.980.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sachin Singh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; timsTOF fleX

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-26 23:43:37	ID requested
⏵ 1	2024-01-26 07:49:34	announced

Lunde NN, Osoble NMM, Fernandez AD, Antobreh AS, Jafari A, Singh S, Nyman TA, Rustan AC, Solberg R, Thoresen GH, Interplay between Cultured Human Osteoblastic and Skeletal Muscle Cells: Effects of Conditioned Media on Glucose and Fatty Acid Metabolism. Biomedicines, 11(11):(2023) [pubmed]

10.3390/biomedicines11112908;

submitter keyword: Energy metabolism

Myokines

Osteoblastic cells

Osteokines

Primary human myotubes;

G. Hege Thoresen
contact affiliation	Section for Pharmacology and Pharmaceutical Biosciences, Department of Pharmacy, University of Oslo,
contact email	hege.thoresen@farmasi.uio.no
lab head
Sachin Singh
contact affiliation	Proteomics core facility, Olso University hospital, Norway
contact email	sachin.kumar.singh@rr-research.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045723
     1. Label: PRIDE project
     2. Name: Interplay between cultured human osteoblastic and skeletal muscle cells: Effects of conditioned media on glucose and fatty acid metabolism