PXD045540

PXD045540 is an original dataset announced via ProteomeXchange.

Title	Proteomics for ferroptosis-related and CYR61-related molecules
Description	This project contains 5 subprojects as to explore role of CYR61 in ferroptosis. In project 1 and 5, lung cancer cells treated with DMSO, RSL3 or erastin for 24 h were collected to perform TMT in order to screen ferroptosis-related molecules. LC-MS/MS in project 2 and 3 were based on immunoprecipitation. anti-CYR61 or anti-ETFA antibodies were used to perform IP with extracted mitochondrial proteins. SAS-PAGE was performed to separate proteins. Gel strips were then cut by panel to perform LC-MS/MS to identify interacted proteins. In project4, blue native-PAGE (BNE) was first applied to separate mitochondrial complexes. Gels strips of target complexes were then isolated to perform LC-MS/MS as to identify components of target complexes.
HostingRepository	iProX
AnnounceDate	2023-09-20
AnnouncementXML	Submission_2023-09-20_23:20:29.869.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wanxin Guo
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Q Exactive Plus; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-20 23:20:14	ID requested
⏵ 1	2023-09-20 23:20:30	announced

Dataset with its publication pending

submitter keyword: Ferroptosis, CYR61, CCN1, BNE, mitochondria, IP

Wanxin Guo
contact affiliation	Shanghai Chest Hospital
contact email	WandaGWX@163.com
lab head
Wanxin Guo
contact affiliation	Shanghai Chest Hospital
contact email	WandaGWX@163.com
dataset submitter

iProX dataset URI