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PXD045525-1

PXD045525 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSecreted footprint of pluripotency in Man-13 HeSCs
DescriptionWith a view to developing a much-needed non-invasive method for monitoring the healthy pluripotent state of human stem cells in culture, we undertook proteomic analysis of the waste medium from cultured induced (Rebl.PAT) human pluripotent stem cells (hPSCs). Cells were grown in E8 medium to maintain pluripotency, and then transferred to FGF2 and TGFβ deficient E6 media for 48 hours to replicate an early, undirected dissolution of pluripotency. We identified a distinct proteomic footprint associated with early loss of pluripotency in both hPSC lines, and a strong correlation with changes in the transcriptome. We demonstrate that multiplexing of four E8- against four E6- enriched secretome biomarkers provides a robust, diagnostic metric for pluripotent state. These biomarkers were further confirmed by Western blotting which demonstrated consistent correlation with the pluripotent state across cell lines, and in response to a recovery assay.
HostingRepositoryPRIDE
AnnounceDate2024-03-26
AnnouncementXMLSubmission_2024-03-26_15:18:55.471.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPhil Lewis
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-09-20 08:33:25ID requested
12024-03-26 15:18:55announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: pluripotency,IPSC, stem cells
Contact List
Susan Kimber
contact affiliationUniversity of Manchester
contact emailsusan.kimber@manchester.ac.uk
lab head
Phil Lewis
contact affiliationUniversity of Bristol
contact emailphil.lewis@bristol.ac.uk
dataset submitter
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Dataset FTP location
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