PXD045186 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chloride intracellular channel (CLIC) proteins function as fusogens |
Description | The Cl- intracellular channel (CLIC) family members uniquely transition between soluble and membrane-associated conformations. Despite decades of extensive functional and structural studies, CLICs’ function as ion channels remains debated, rendering our understanding of their physiological role incomplete. Here, we expose a novel function of CLIC5 as a fusogen. We demonstrate that purified CLIC5 directly interacts with the membrane and induces fusion, as reflected by increased liposomal diameter and lipid and content mixing between liposomes. Moreover, we show that this activity is facilitated by acidic pH, a known trigger for CLICs’ transition to a membrane-associated conformation and that increased exposure of the hydrophobic inter-domain interface is crucial for this process. Finally, mutation of a conserved hydrophobic interfacial residue diminishes the fusogenic activity of CLIC5 in vitro and impairs excretory canal formation in C. elegans in vivo. Together, our results unravel the long-sought physiological role of these enigmatic proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2024-03-10 |
AnnouncementXML | Submission_2024-03-10_04:00:06.809.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Pavla Vankova |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-07 02:44:14 | ID requested | |
⏵ 1 | 2024-03-10 04:00:07 | announced | |
Publication List
Keyword List
ProteomeXchange project tag: Hydrogen Deuterium Exchange (HDX-MS) |
submitter keyword: CLIC protein family, fusogenic activity, CLIC5, HDX-MS |
Contact List
Dr Petr Man |
contact affiliation | Laboratory of Structural Biology and Cell Signaling Institute of Microbiology of the Czech Acad Sci Prumyslova 595 252 50 Vestec Czech Republic |
contact email | pman@biomed.cas.cz |
lab head | |
Pavla Vankova |
contact affiliation | Institute of Biotechnology of the Czech Academy of Sciences, BioCeV, Prumyslova 595, 252 50 Vestec, Czech Republic |
contact email | pavla.vankova@ibt.cas.cz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045186
- Label: PRIDE project
- Name: Chloride intracellular channel (CLIC) proteins function as fusogens