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PXD044865-1

PXD044865 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe phosphorylation landscape of infection-related development by the rice blast fungus
DescriptionMany of the world’s most devastating crop diseases are caused by fungal pathogens which elaborate specialized infection structures to invade plant tissue. Here we present a quantitative mass spectrometry-based phosphoproteomic analysis of infection-related development by the rice blast fungus Magnaporthe oryzae, which threatens global food security. We mapped 8,005 phosphosites on 2,062 fungal proteins, revealing major re-wiring of phosphorylation-based signaling cascades during fungal infection. Comparingme phosphosite conservation across 41 fungal species reveals phosphorylation signatures specifically associated with biotrophic and hemibiotrophic fungal infection. We then used parallel reaction monitoring to identify phosphoproteins directly regulated by the Pmk1 MAP kinase that controls plant infection by M. oryzae. We define 33 substrates of Pmk1 and show that Pmk1-dependent phosphorylation of a newly identified regulator, Vts1, is required for rice blast disease. Defining the phosphorylation landscape of infection therefore identifies potential therapeutic interventions for control of plant diseases.
HostingRepositoryPanoramaPublic
AnnounceDate2024-04-03
AnnouncementXMLSubmission_2024-04-03_08:57:41.378.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportsupported by repository but incomplete data and/or metadata
PrimarySubmitterFrank Menke
SpeciesList scientific name: Pyricularia oryzae; NCBI TaxID: 318829;
ModificationListOxidation; Phospho
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-25 12:07:52ID requested
12024-04-03 08:57:41announced
Publication List
Cruz-Mireles N, Osés-Ruiz M, Derbyshire P, Jégousse C, Ryder LS, Bautista MJ, Eseola AB, Sklenar J, Tang B, Yan X, Ma W, Findlay KC, Were V, MacLean D, Talbot NJ, Menke FLH. The phosphorylation landscape of infection-related development by the rice blast fungus. bioRxiv [Internet]. 2023 Jan 1;2023.08.19.553964. Available from: http://biorxiv.org/content/early/2023/08/20/2023.08.19.553964.abstract
Keyword List
submitter keyword: Fungus, Appressorium, Pmk1 pathway, protein phosphorylation, phosphoproteomics
Contact List
Frank Menke
contact affiliationThe Sainsbury Laboratory
contact emailfrank.menke@tsl.ac.uk
lab head
Frank Menke
contact affiliationThe Sainsbury Laboratory
contact emailfrank.menke@tsl.ac.uk
dataset submitter
Full Dataset Link List
Panorama Public dataset URI