PXD044865

PXD044865 is an original dataset announced via ProteomeXchange.

Title	The phosphorylation landscape of infection-related development by the rice blast fungus
Description	Many of the world’s most devastating crop diseases are caused by fungal pathogens which elaborate specialized infection structures to invade plant tissue. Here we present a quantitative mass spectrometry-based phosphoproteomic analysis of infection-related development by the rice blast fungus Magnaporthe oryzae, which threatens global food security. We mapped 8,005 phosphosites on 2,062 fungal proteins, revealing major re-wiring of phosphorylation-based signaling cascades during fungal infection. Comparingme phosphosite conservation across 41 fungal species reveals phosphorylation signatures specifically associated with biotrophic and hemibiotrophic fungal infection. We then used parallel reaction monitoring to identify phosphoproteins directly regulated by the Pmk1 MAP kinase that controls plant infection by M. oryzae. We define 33 substrates of Pmk1 and show that Pmk1-dependent phosphorylation of a newly identified regulator, Vts1, is required for rice blast disease. Defining the phosphorylation landscape of infection therefore identifies potential therapeutic interventions for control of plant diseases.
HostingRepository	PanoramaPublic
AnnounceDate	2024-04-03
AnnouncementXML	Submission_2024-04-03_08:57:41.378.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	supported by repository but incomplete data and/or metadata
PrimarySubmitter	Frank Menke
SpeciesList	scientific name: Pyricularia oryzae; NCBI TaxID: 318829;
ModificationList	Oxidation; Phospho
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-25 12:07:52	ID requested
⏵ 1	2024-04-03 08:57:41	announced

Cruz-Mireles N, Osés-Ruiz M, Derbyshire P, Jégousse C, Ryder LS, Bautista MJ, Eseola AB, Sklenar J, Tang B, Yan X, Ma W, Findlay KC, Were V, MacLean D, Talbot NJ, Menke FLH. The phosphorylation landscape of infection-related development by the rice blast fungus. bioRxiv [Internet]. 2023 Jan 1;2023.08.19.553964. Available from: http://biorxiv.org/content/early/2023/08/20/2023.08.19.553964.abstract

submitter keyword: Fungus, Appressorium, Pmk1 pathway, protein phosphorylation, phosphoproteomics

Frank Menke
contact affiliation	The Sainsbury Laboratory
contact email	frank.menke@tsl.ac.uk
lab head
Frank Menke
contact affiliation	The Sainsbury Laboratory
contact email	frank.menke@tsl.ac.uk
dataset submitter

Panorama Public dataset URI