PXD044578

PXD044578 is an original dataset announced via ProteomeXchange.

Title	Suppression and counter-suppression of plant immunity by an exo-1,3-glucanase of GH17 family and an elongation factor 1 of the rice blast fungus
Description	Fungal cell walls undergo continual remodeling during hyphal growth, development, infection and adaptation to environmental stress. Cell wall remodeling generates 1,3-glucan fragments by diverse endo-glycosyl hydrolases (GH), which are well-known pathogen-associated molecular patterns (PAMPs). How fungal pathogens evade plant immunity triggered by1,3-glucan fragments and associated GH proteins is not known. Here, we report a novel mechanism of immune evasion underlying the suppression of 1,3-glucan-triggered plant immunity by the blast fungus Magnaporthe oryzae. An exo-1,3-glucanase of the GH17 family, named Ebg1, is important for fungal cell wall integrity and virulence of M. oryzae. Ebg1 can hydrolyze 1,3-glucan and laminarin into glucose to prevent 1,3-glucan-triggered plant immunity, but also acts as a PAMP, independent of its hydrolase activity. Surprisingly, M. oryzae engages an elongation factor 1 alpha protein (EF1 to interact and co-localize with Ebg1 in the apoplast to suppress Ebg1-triggered immunity. Since both Ebg1 and EF1 are widely distributed in fungi, their interaction may be a conserved mechanism whereby fungal pathogens evade plant immunity and safeguard cell wall remodeling during infection.
HostingRepository	iProX
AnnounceDate	2023-08-14
AnnouncementXML	Submission_2024-02-21_18:43:22.449.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ning Liu
SpeciesList	scientific name: Magnaporthe; NCBI TaxID: 148303;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-14 19:29:45	ID requested
1	2023-08-14 19:30:25	announced
⏵ 2	2024-02-21 18:43:23	announced	2024-02-22: Update Information

Liu H, Lu X, Li M, Lun Z, Yan X, Yin C, Yuan G, Wang X, Liu N, Liu D, Wu M, Luo Z, Zhang Y, Bhadauria V, Yang J, Talbot NJ, Peng YL, of the rice blast fungus. Nat Commun, 14(1):5491(2023) [pubmed]

submitter keyword: magnaporthe, barley, apoplastic fluid

Xunli Lu
contact affiliation	China Agricultural University
contact email	luxunli@cau.edu.cn
lab head
Ning Liu
contact affiliation	China Agricultural University
contact email	liuning@cau.edu.cn
dataset submitter

iProX dataset URI