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PXD044470-1

PXD044470 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe selfish yeast plasmid exploits a SWI/SNF-type chromatin remodeling complex for hitchhiking on chromosomes and ensuring high-fidelity propagation
DescriptionExtra-chromosomal selfish DNA elements can evade the risk of being lost at every generation by behaving as chromosome appendages, thereby ensuring high fidelity segregation and stable persistence in host cell populations. The yeast 2-micron plasmid and episomes of the mammalian gammaherpes and papilloma viruses that tether to chromosomes and segregate by hitchhiking on them exemplify this strategy. We document for the first time the utilization of a SWI/SNF-type chromatin remodeling complex as a conduit for chromosome association by a selfish element. One principal mechanism for chromosome tethering by the 2-micron plasmid is the bridging interaction of the plasmid partitioning proteins (Rep1 and Rep2) with the yeast RSC2 complex and the plasmid partitioning locus STB. We substantiate this model by multiple lines of evidence derived from genomics, cell biology and interaction analyses. We describe a Rep-STB bypass system in which a plasmid engineered to non-covalently associate with the RSC complex mimics segregation by chromosome hitchhiking. Given the ubiquitous prevalence of SWI/SNF family chromatin remodeling complexes among eukaryotes, it is likely that the 2-micron plasmid paradigm or analogous ones will be encountered among other eukaryotic selfish elements.
HostingRepositoryPRIDE
AnnounceDate2023-08-24
AnnouncementXMLSubmission_2023-08-24_08:35:18.568.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIyerlabMember
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-09 13:56:45ID requested
12023-08-24 08:35:18announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: RSC2
2-micron plasmid
chromatin remodeling
selfish DNA
chromosome-hitchhiking
Contact List
VishwanathIyer
contact affiliationDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA Livestrong Cancer Institutes and Department of Oncology, Dell Medical School, The University of Texas at Austin, Austin, TX, USA
contact emailiyerlab@gmail.com
lab head
IyerlabMember
contact affiliationThe University of Texas at Austin
contact emailiyerlab@gmail.com
dataset submitter
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Dataset FTP location
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