PXD043253

PXD043253 is an original dataset announced via ProteomeXchange.

Title	Pseudomonas aeruginosa MipA-MipB envelope proteins are new sensors of polymyxin
Description	Due to the rising incidence of antibiotic resistant infections, last-line antibiotics polymyxins have resurged in the clinics together with the appearance of new bacterial strategies of escape. The Gram-negative opportunistic pathogen Pseudomonas aeruginosa develops resistance to colistin/polymyxin by distinct molecular mechanisms, mostly involving the modification of the lipid A component of the LPS by proteins encoded within the arnBCDATEF-ugD (called arn) operon. We characterized a polymyxin-induced operon, named mipBA, present in P. aeruginosa group of strains devoid of the arn operon. Mass spectrometry-based quantitative proteomics showed that the absence of MipBA modifies the membrane proteome, notably impacting ParRS-regulated proteins, in response to polymyxin.
HostingRepository	PRIDE
AnnounceDate	2024-01-12
AnnouncementXML	Submission_2024-01-12_01:08:44.524.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043253
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-23 15:29:37	ID requested
⏵ 1	2024-01-12 01:08:44	announced

10.6019/PXD043253;

submitter keyword: antibiotics, resistance, mipBA operon, polymyxins,Pseudomonas aeruginosa, nanoLC-MS/MS

Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD043253

PRIDE project URI

• PRIDE
  1. PXD043253
     1. Label: PRIDE project
     2. Name: Pseudomonas aeruginosa MipA-MipB envelope proteins are new sensors of polymyxin