PXD042990 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Regulation of transcript abundance and translationny decapping activators Pat1 and Dhh1 |
Description | We have examined the roles of yeast mRNA decapping-activators Pat1 and Dhh1 in repressing the translation and abundance of specific mRNAs in nutrient-replete cells using ribosome profiling, RNA-Seq, CAGE analysis of capped mRNAs, RNA Polymerase II ChIP-Seq, and TMT-mass spectrometry of mutants lacking one or both factors. Although the Environmental Stress Response (ESR) is activated in dhh1 and pat1 mutants, hundreds of non-ESR transcripts are elevated in a manner indicating cumulative repression by Pat1 and Dhh1 in WT. These mRNAs show reduced decapping and diminished transcription in the mutants, indicating that impaired mRNA turnover drives transcript derepression in cells lacking Dhh1 or Pat1. mRNA degradation stimulated by Dhh1/Pat1 is not dictated by poor translation nor enrichment for suboptimal codons. Pat1 and Dhh1 also collaborate to reduce translation and protein production from many mRNAs. Transcripts showing concerted translational repression by Pat1/Dhh1 include mRNAs involved in cell adhesion or utilization of the poor nitrogen source allantoin. Pat1/Dhh1 also repress numerous transcripts involved in respiration, catabolism of non-preferred carbon or nitrogen sources, or autophagy; and we obtained evidence for elevated respiration and autophagy in the mutants. Thus, Pat1 and Dhh1 function as post-transcriptional repressors of multiple pathways normally activated only during nutrient limitation. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:11:40.017.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD042990 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Anil Kumar Vijjamarri |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-06-14 11:35:51 | ID requested | |
1 | 2023-10-24 13:32:26 | announced | |
⏵ 2 | 2023-11-14 09:11:42 | announced | 2023-11-14: Updated project metadata. |
Publication List
Vijjamarri AK, Gupta N, Onu C, Niu X, Zhang F, Kumar R, Lin Z, Greenberg ML, Hinnebusch AG, mRNA decapping activators Pat1 and Dhh1 regulate transcript abundance and translation to tune cellular responses to nutrient availability. Nucleic Acids Res, 51(17):9314-9336(2023) [pubmed] |
10.6019/PXD042990; |
Keyword List
submitter keyword: mRNA decay,S. cerevesiea, translation |
Contact List
Alan G Hinnebusch |
contact affiliation | Head, Section on Nutrient Control of Gene Expression Building 6, Room 230 6 Center Drive Bethesda, MD 20892 Phone: 301-496-4480 |
contact email | alanh@mail.nih.gov |
lab head | |
Anil Kumar Vijjamarri |
contact affiliation | National Institutes of Health |
contact email | anilkumar.vijjamarri@nih.gov |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042990
- Label: PRIDE project
- Name: Regulation of transcript abundance and translationny decapping activators Pat1 and Dhh1