PXD042977-1
PXD042977 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | The proteome data of extracellular vesicles derived from human umbilical cord and bone marrow mesenchymal stem cells. |
Description | The protein samples stored at -80 °C were thawed and treated with lysis buffer (8M urea, 1% protease inhibitor, 50mM NH4HCO3) for 5 minutes in room temperature. After ultrasonic cracking on ice and centrifugation at 14000g for 10 min at 20 °C, the amount of total protein of each MSC sample was detected by bicinchoninic acid assay (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) using NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, Inc., Sunnyvale, CA, USA). Protein digestion was carried out by a filter-aided sample preparation technique. Briefly, 50 μg of a sample was loaded onto a Microcon filter unit YM-30 (Millipore) and washed with 8 M urea, followed by protein alkylation with 50 mM iodoacetamide in 8 M urea in darkness for 20 min. After alkylation, the filters were washed twice with 8 M urea and twice with 40 mM ammonium bicarbonate. Trypsin (Promega, Madison, WI, USA) was added to the samples at 100:1 protein-to-enzyme ratio and incubated overnight at 37 °C. Later the same |
HostingRepository | iProX |
AnnounceDate | 2023-06-12 |
AnnouncementXML | Submission_2023-06-14_04:11:45.145.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yuchen Gao |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-06-14 04:11:36 | ID requested | |
⏵ 1 | 2023-06-14 04:11:45 | announced | |
2 | 2023-08-29 21:42:45 | announced | 2023-08-30: Update publication information. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: mesenchymal stem cell, senescence |
Contact List
Lei Zhang | |
---|---|
contact affiliation | State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College |
contact email | zhanglei1@ihcams.ac.cn |
lab head | |
Yuchen Gao | |
contact affiliation | State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China |
contact email | 821120396@qq.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |