<<< Full experiment listing

PXD042770-1

PXD042770 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA comprehensive spectral assay library to quantify the Halobacterium salinarum NRC-1 proteome by DIA/SWATH-MS
DescriptionData-Independent Acquisition (DIA) is a mass spectrometry-based method to reliably identify and reproducibly quantify large fractions of a target proteome. The peptide-centric data analysis strategy employed in DIA requires a priori generated spectral assay libraries. Such assay libraries allow to extract quantitative data in a targeted approach and have been generated for human, mouse, zebrafish, E. coli and few other organisms. However, a spectral assay library for the extreme halophilic archaeon Halobacterium salinarum NRC-1, a model organism that contributed to several notable discoveries, is not publicly available yet. Here, we report a comprehensive spectral assay library to measure 2,563 of 2,646 annotated H. salinarum NRC-1 proteins. We demonstrate the utility of this library by measuring global protein abundances over time under standard growth conditions. The H. salinarum NRC-1 library includes 21,074 distinct peptides representing 97% of the predicted proteome and provides a new, valuable resource to confidently measure and quantify any protein of this archaeon.
HostingRepositoryPRIDE
AnnounceDate2023-09-21
AnnouncementXMLSubmission_2023-09-21_00:37:09.915.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterUlrikeKusebauch
SpeciesList scientific name: Halobacterium salinarum; NCBI TaxID: 2242;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-06 11:42:15ID requested
12023-09-21 00:37:10announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: DIA, Data-independent acquisition, SWATH,Halobacterium salinarum NRC-1, spectral assay library, DDA, data-dependent acquisition
Contact List
RobertL.
contact affiliationInstitute for Systems Biology, Seattle, WA, USA
contact emailrmoritz@systemsbiology.org
lab head
UlrikeKusebauch
contact affiliationInstitute for Systems Biology, Seattle, WA, USA
contact emailukusebauch@systemsbiology.org
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD042770
PRIDE project URI
Repository Record List
[ + ]