PXD042770

PXD042770 is an original dataset announced via ProteomeXchange.

Title	A comprehensive spectral assay library to quantify the Halobacterium salinarum NRC-1 proteome by DIA/SWATH-MS
Description	Data-Independent Acquisition (DIA) is a mass spectrometry-based method to reliably identify and reproducibly quantify large fractions of a target proteome. The peptide-centric data analysis strategy employed in DIA requires a priori generated spectral assay libraries. Such assay libraries allow to extract quantitative data in a targeted approach and have been generated for human, mouse, zebrafish, E. coli and few other organisms. However, a spectral assay library for the extreme halophilic archaeon Halobacterium salinarum NRC-1, a model organism that contributed to several notable discoveries, is not publicly available yet. Here, we report a comprehensive spectral assay library to measure 2,563 of 2,646 annotated H. salinarum NRC-1 proteins. We demonstrate the utility of this library by measuring global protein abundances over time under standard growth conditions. The H. salinarum NRC-1 library includes 21,074 distinct peptides representing 97% of the predicted proteome and provides a new, valuable resource to confidently measure and quantify any protein of this archaeon.
HostingRepository	PRIDE
AnnounceDate	2023-09-21
AnnouncementXML	Submission_2023-09-21_00:37:09.915.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	UlrikeKusebauch
SpeciesList	scientific name: Halobacterium salinarum; NCBI TaxID: 2242;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-06 11:42:15	ID requested
⏵ 1	2023-09-21 00:37:10	announced

Dataset with its publication pending

submitter keyword: DIA, Data-independent acquisition, SWATH,Halobacterium salinarum NRC-1, spectral assay library, DDA, data-dependent acquisition

RobertL.
contact affiliation	Institute for Systems Biology, Seattle, WA, USA
contact email	rmoritz@systemsbiology.org
lab head
UlrikeKusebauch
contact affiliation	Institute for Systems Biology, Seattle, WA, USA
contact email	ukusebauch@systemsbiology.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD042770

PRIDE project URI

• PRIDE
  1. PXD042770
     1. Label: PRIDE project
     2. Name: A comprehensive spectral assay library to quantify the Halobacterium salinarum NRC-1 proteome by DIA/SWATH-MS