PXD042729

PXD042729 is an original dataset announced via ProteomeXchange.

Title	Proteomics-Based Identification of Retinal Protein Networks Impacted by Elevated Intraocular Pressure in the Hypertonic Saline Injection Model of Experimental Glaucoma
Description	Elevated intraocular pressure is considered a major cause of glaucomatous retinal neurodegeneration. To facilitate a better understanding of the underlying molecular processes and mechanisms, we report a study focusing on alterations of the retina proteome by induced ocular hypertension in a rat model of the disease. Glaucomatous processes were modelled through sclerosing the aqueous outflow routes of the eyes by hypertonic saline injections into an episcleral vein. Mass spectrometry-based quantitative retina proteomics using a label-free shotgun methodology identified over 200 proteins significantly affected by ocular hypertension. Various facets of glaucomatous pathophysiology were revealed through the organization of the findings into protein interaction networks and by pathway analyses. Concentrating on retinal neurodegeneration as a characteristic process of the disease, elevated intraocular pressure-induced alterations in the expression of selected proteins were verified by targeted proteomics based on nanoflow liquid chromatography coupled with nanoelectrospray ionization tandem mass spectrometry using the parallel reaction monitoring method of data acquisition. Acquired raw data are shared through deposition to the ProteomeXchange Consortium making a retina proteomics dataset on the selected animal model of glaucoma available for the first time.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:10:40.436.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD042729
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Laszlo Prokai
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-05 08:19:45	ID requested
1	2023-10-24 13:07:39	announced
⏵ 2	2023-11-14 09:10:41	announced	2023-11-14: Updated project metadata.

10.6019/PXD042729;

Zaman K, Nguyen V, Prokai-Tatrai K, Prokai L, Proteomics-Based Identification of Retinal Protein Networks Impacted by Elevated Intraocular Pressure in the Hypertonic Saline Injection Model of Experimental Glaucoma. Int J Mol Sci, 24(16):(2023) [pubmed]

submitter keyword: retina proteomics

glaucoma

ocular hypertension

Brown Norway rat model

bioinformatics

pathway analysis

protein networks

Laszlo Prokai
contact affiliation	Professor and Robert A. Welch Chair in Biochemistry Department of Pharmacology and Neuroscience University of North Texas, Health Science Center
contact email	Laszlo.Prokai@unthsc.edu
lab head
Laszlo Prokai
contact affiliation	Department of Pharmacology & Neuroscience UNT Health Science Center Fort Worth, TX, USA
contact email	advancedms@unthsc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042729
     1. Label: PRIDE project
     2. Name: Proteomics-Based Identification of Retinal Protein Networks Impacted by Elevated Intraocular Pressure in the Hypertonic Saline Injection Model of Experimental Glaucoma