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PXD042439-1

PXD042439 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRobust and scalable fluorosequencing technology
DescriptionTitle: Robust and scalable fluorosequencing technology Corresponding Author: Jagannath Swaminathan HLA peptides from mono-allelic B-cell line using tandem mass-spectrometry We purchased the monoallelic B-cell line (B721.221), with HLA allele A*2603, directly from the International Histocompatibility Working group (Seattle, Washington). We cultured the cells and isolated HLA peptides from approximately 300 million cells as previously described (Abelin et al. 2017). We identified the isolated HLA peptides using an LC-coupled tandem mass-spectrometer (ThermoFisher, Orbitrap Fusion Lumos) and a reference dataset of a human proteome (Swissprot), applying settings from the literature for analyzing HLA peptides (Bassani-Sternberg et al. 2015) and ProteomeDiscoverer 2.3 software (Thermofisher).
HostingRepositoryMassIVE
AnnounceDate2023-08-08
AnnouncementXMLSubmission_2023-08-08_09:28:51.400.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJagannath Swaminathan
SpeciesList scientific name: Human;
ModificationListCarbamidomethyl
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-05-23 10:19:34ID requested
12023-08-08 09:28:51announced
Publication List
no publication
Keyword List
submitter keyword: HLA, Human leukocyte antigen, single molecule protein sequencing
Contact List
Jagannath Swaminathan
contact affiliationErisyon Inc
contact emailjagannath@erisyon.com
lab head
Jagannath Swaminathan
contact affiliationErisyon Inc
contact emailjagannath@erisyon.com
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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