PXD042435 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chromatin proteome of S-phase in IgG- and DONSON-depleted egg extract |
Description | The aim of the project was to analyse the role of DONSON during DNA replication reaction in Xenopus laevis egg extract. To test what is the impact of DONSON on replication we decided to remove DONSON from egg extract by immunodepletion and analyse its impact on DNA replication reaction. In the parallel experiments we have shown that without DONSON egg extract cannot synthesise nascent DNA. Here we wanted to examine the change of replicating chromatin proteome in absence of DONSON. Interphase Xenopus laevis egg extract was immunodepleted with control nonspecific IgG antibodies beads or DONSON-antibodies. The efficiency of immunodepletion was shown to be over 95%. DNA replication was set up in both IgG- and DONSON-depleted egg extracts and chromatin isolated in the middle of S-phase, when replication forks where abundant in chromatin fraction in IgG-depleted extract. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:10:39.305.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD042435 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Aga Gambus |
SpeciesList | scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-05-23 07:28:53 | ID requested | |
1 | 2023-10-24 12:59:52 | announced | |
⏵ 2 | 2023-11-14 09:10:48 | announced | 2023-11-14: Updated project metadata. |
Publication List
10.6019/PXD042435; |
Kingsley G, Skagia A, Passaretti P, Fernandez-Cuesta C, Reynolds-Winczura A, Koscielniak K, Gambus A, DONSON facilitates Cdc45 and GINS chromatin association and is essential for DNA replication initiation. Nucleic Acids Res, 51(18):9748-9763(2023) [pubmed] |
Keyword List
submitter keyword: replication initiation, Xenopus,DONSON, chromatin |
Contact List
Dr Aga Gambus |
contact affiliation | Dr Aga Gambus, Reader in Genome Replication, Wellcome and Lister Fellow, Chromosomal Replication Laboratory, Institute for Cancer and Genomic Sciences, IBR west, WX2.67, University of Birmingham, Edgbaston, Birmingham, B15 2TT |
contact email | a.gambus@bham.ac.uk |
lab head | |
Aga Gambus |
contact affiliation | University of Birmingham |
contact email | a.gambus@bham.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042435
- Label: PRIDE project
- Name: Chromatin proteome of S-phase in IgG- and DONSON-depleted egg extract