PXD042435

PXD042435 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Chromatin proteome of S-phase in IgG- and DONSON-depleted egg extract
Description	The aim of the project was to analyse the role of DONSON during DNA replication reaction in Xenopus laevis egg extract. To test what is the impact of DONSON on replication we decided to remove DONSON from egg extract by immunodepletion and analyse its impact on DNA replication reaction. In the parallel experiments we have shown that without DONSON egg extract cannot synthesise nascent DNA. Here we wanted to examine the change of replicating chromatin proteome in absence of DONSON. Interphase Xenopus laevis egg extract was immunodepleted with control nonspecific IgG antibodies beads or DONSON-antibodies. The efficiency of immunodepletion was shown to be over 95%. DNA replication was set up in both IgG- and DONSON-depleted egg extracts and chromatin isolated in the middle of S-phase, when replication forks where abundant in chromatin fraction in IgG-depleted extract.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:10:39.305.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD042435
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Aga Gambus
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-23 07:28:53	ID requested
1	2023-10-24 12:59:52	announced
⏵ 2	2023-11-14 09:10:48	announced	2023-11-14: Updated project metadata.

Publication List

10.6019/PXD042435;
Kingsley G, Skagia A, Passaretti P, Fernandez-Cuesta C, Reynolds-Winczura A, Koscielniak K, Gambus A, DONSON facilitates Cdc45 and GINS chromatin association and is essential for DNA replication initiation. Nucleic Acids Res, 51(18):9748-9763(2023) [pubmed]

10.6019/PXD042435;

Kingsley G, Skagia A, Passaretti P, Fernandez-Cuesta C, Reynolds-Winczura A, Koscielniak K, Gambus A, DONSON facilitates Cdc45 and GINS chromatin association and is essential for DNA replication initiation. Nucleic Acids Res, 51(18):9748-9763(2023) [pubmed]

Keyword List

submitter keyword: replication initiation, Xenopus,DONSON, chromatin

submitter keyword: replication initiation, Xenopus,DONSON, chromatin

Contact List

Dr Aga Gambus
contact affiliation	Dr Aga Gambus, Reader in Genome Replication, Wellcome and Lister Fellow, Chromosomal Replication Laboratory, Institute for Cancer and Genomic Sciences, IBR west, WX2.67, University of Birmingham, Edgbaston, Birmingham, B15 2TT
contact email	a.gambus@bham.ac.uk
lab head
Aga Gambus
contact affiliation	University of Birmingham
contact email	a.gambus@bham.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD042435
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD042435

PRIDE project URI

Repository Record List

[ + ]