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PXD042358-1

PXD042358 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of membrane proteins regulated by ADAM15 by SUSPECS proteomics
DescriptionADAM15 is a member of the disintegrin-metalloproteinase family of sheddases, which plays a role in several biological processes including cartilage homeostasis. In contrast with well-characterized ADAMs, such as the canonical sheddases ADAM17 and ADAM10, little is known about substrates of ADAM15 or how the enzyme exerts its biological functions. Herein, we used “surface-spanning enrichment with click-sugars (SUSPECS)” proteomics to identify ADAM15 substrates and/or proteins regulated by the proteinase at the cell surface of chondrocyte-like cells. Silencing of ADAM15 by siRNAs significantly altered membrane levels of 13 proteins, all previously not known to be regulated by ADAM15. We used orthogonal techniques to validate ADAM15 effects on 3 of these proteins which have known roles in cartilage homeostasis. This confirmed that ADAM15-silencing increased cell surface levels of the programmed cell death 1 ligand 2 (PDCD1LG2) and reduced cell surface levels of vasorin and the sulfate transporter SLC26A2 through an unknown post-translational mechanism. The increase of PDCD1LG2 by ADAM15 knockdown, a single-pass type I transmembrane protein, suggested it could be a proteinase substrate. However, shed PDCD1LG2 could not be detected even by a data-independent acquisition mass spectrometry, a highly sensitive method for identification and quantification of proteins in complex protein samples, suggesting that ADAM15 regulates PDCD1LG2 membrane levels by a mechanism different from ectodomain shedding.
HostingRepositoryPRIDE
AnnounceDate2023-06-13
AnnouncementXMLSubmission_2023-06-13_06:16:32.643.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMatteoCalligaris
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-05-19 05:50:49ID requested
12023-06-13 06:16:33announced
22023-11-14 08:57:21announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: chondrocytes, proteomics, osteoarthritis, mass spectrometry-LC-MS/MS,Adams, SPECS, ADAM15, metalloproteases
Contact List
Simone DarioScilabra
contact affiliationFondazione Ri.MED
contact emailsdscilabra@fondazionerimed.com
lab head
MatteoCalligaris
contact affiliationFondazione Ri.MED
contact emailmcalligaris@fondazionerimed.com
dataset submitter
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Dataset FTP location
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