PXD041610 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proximity labeling in Arabidopsis seedlings expressing R-degron-Turbo vs. M-degron-Turbo vs. L-degron-Turbo untreated (DMSO control), treated with 10µM Bortezomib and treated with 1µM Concanamycin A. |
Description | Goal was to identify N-recognins for Arginine and Leucine by comparison of biotinylated proteins from 1 week old whole Arabidopsis seedlings constitutively expressing the bait constructs R-degron-Turbo or L-degron-Turbo. M-degron-Turbo served as a negative control. The plants were treated with 50µM biotin and either the proteasome inhibitor Bortezomib (10µM) or the inhibitor of vacuolar acidification Concanamycin A (1µM) or DMSO one hour before harvest. |
HostingRepository | PRIDE |
AnnounceDate | 2024-04-15 |
AnnouncementXML | Submission_2024-04-15_04:46:19.218.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | WeiQiang Chen |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | biotinylated residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-04-17 23:41:26 | ID requested | |
⏵ 1 | 2024-04-15 04:46:19 | announced | |
Publication List
Keyword List
submitter keyword: Bortezomib,N-degron pathway, proximity labeling, Concanamycin A, protein turnover, Turbo |
Contact List
Andreas Bachmair |
contact affiliation | Max Perutz Labs, University of Vienna |
contact email | andreas.bachmair@univie.ac.at |
lab head | |
WeiQiang Chen |
contact affiliation | Mass Spectrometry Facility, Max Perutz Laboratories Support GmbH |
contact email | chenweiqiang@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD041610
- Label: PRIDE project
- Name: Proximity labeling in Arabidopsis seedlings expressing R-degron-Turbo vs. M-degron-Turbo vs. L-degron-Turbo untreated (DMSO control), treated with 10µM Bortezomib and treated with 1µM Concanamycin A.